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Bioprocess Development
We empower you on your bioprocess development journey
Upstream bioprocess development aims to maximize the yield and quality of the desired end product while reducing development time and production costs. Our bioprocessing solutions help you achieve these goals. Eppendorf supports you on your bioprocess development journey by providing bioprocess hardware, software solutions, and services, all orchestrated to accelerate upstream process development, streamline scale-up, and harness the power of data. Read on to learn more about them and to get some inspiration for advancing bioprocess development .
Let’s talk development. Let’s talk progress. We're here to help you develop strategies for your bioprocess development journey!
Let’s talk development. Let’s talk progress. We're here to help you develop strategies for your bioprocess development journey!
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Identify critical process parameters
Automate tasks
Enable your team
From R&D to production
Secure batches
Let’s talk development. Let’s talk progress. We're here to help you find inspiration to identify critical process parameters faster.
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I would like to know how to monitor and control bioprocessing parameters.
Visit our bioprocess monitoring and control web page to learn how to monitor bioprocess parameters, which parameters are relevant, and how to control process parameters using feedback loops.
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I need to know which bioprocess parameters are relevant and which setpoints I should use for my upstream development project.
The critical process parameters and setpoints will depend on the type of organism you cultivate. Visit our website for more detailed information about cell culture in bioreactors , stem cell bioprocessing , bacterial fermentation , and yeast fermentation .
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I need to test different experimental conditions. How can I do it more quickly?
A good way to speed up bioprocess development is the use of parallel bioprocess systems like the DASbox Mini Bioreactor System or the DASGIP Parallel Bioreactor System . They facilitate multiple experiments to be run simultaneously and like this saving time and resources. Are you interested in an example? Then have a look at this application note from the Zuerich University for Applied Sciences . The researchers used a DASbox Mini Bioreactor System to run four CHO cell culture runs in parallel and reached highly reproducible results.
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I would like to know more about DoE.
The use of parallel bioprocessing enables a Design of Experiments (DoE) approach, which aims to uncover the complex relationships between process parameters in bioprocess development, through altering a number of variables simultaneously. Not only does this provide fundamental details on a variety of parameters, it also analyzes their potential interactions, and identifies which parameters have the largest effects on the final outcome. Combining DoE and parallel bioprocessing provides a powerful and efficient way to optimize cell cultivation using a minimal number of experiments.
Find out more about the DoE approach in our article:
Find out more about the DoE approach in our article:
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I'd like to see an example of how to systematically optimize process parameters and the benefits of doing so.
For a great example of systematic optimization of process parameters in bioprocess development, we recommend you take a look at this application note from a team at the Medical University of Hannover . It describes how systematic optimization of process parameters, including pH, DO and feeding strategy, led to a more than 10-fold increase in cell density compared to uncontrolled conditions.
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How can AI-based tools help increase efficiency in bioprocess development?
A wide variety of sensors and analyzers generate a vast amount of bioprocess data. The challenge is to efficiently analyze the data and turn it into insights. AI-based tools can help increase efficiency in bioprocess development by reducing the experimental effort per project, reducing time and cost, and increasing development capacity. They help increase the effectiveness of bioprocess development by providing better insights and process knowledge, enabling higher productivity and the development of more robust processes. For further reading, we recommend the resources of DataHow , a company at the forefront of applying a new generation of AI technologies to bioprocessing and one of our partners. Find information on how AI-based tools can reduce wet-lab experiments , what is model-based upstream process optimization , and more.
Find out how the Eppendorf BioNsight cloud software solutions streamlines the use of your bioprocess data for the analysis with DataHow’s AI-based tools.
Find out how the Eppendorf BioNsight cloud software solutions streamlines the use of your bioprocess data for the analysis with DataHow’s AI-based tools.
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Automate routine tasks in bioprocess development to focus on your valuable research
Automating routine tasks allows scientists to focus on valuable research and reduces the risk of human error. Our solutions empower you to increase bioprocess development efficiency through automating manual steps in the laboratory, process control, and data handling.
- Automate sampling: The Bioprocess Autosampler facilitates sampling 24/7 in regular, short intervals, helping you obtain strong datasets and freeing up time for other tasks.
- Automate bioprocess control: DASware control software enables you to automate process control by implementing tailored feedback loops to reduce manual work and make process control more precise.
- Automate data handling: DASware control software and BioNsight cloud provide automated data management in a central location for easy access from anywhere and in-depth analysis.
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Let’s talk development. Let’s talk progress. We're here to help you find inspiration how to automate tasks in bioprocess development.
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I'd like to see an example of how automated sampling can reduce manual labor in bioprocess development.
For an example of automated bioprocess sampling, we recommend you take a look at this application note from one of our customers . It describes how the Bioprocess Autosampler from Eppendorf took samples from eight bioreactors which were operated in parallel, at various time points during an E. coli fermentation run.
If you are interested in automated bioreactor sampling in cell culture bioprocesses, then take a look at this application note from our lab.
If you are interested in automated bioreactor sampling in cell culture bioprocesses, then take a look at this application note from our lab.
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I would like to use process analytical technology to reduce manual labor and automate process control. Where can I find some examples?
Visit our website on process analytical technology to find application examples, in which the use of PAT and automated process control improved the bioprocess by automating glucose control, monitoring cell density, or monitoring the product.
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Enable your team to perform at their best
Parallel and scalable bioreactor systems, single-use bioreactors, and software solutions are all tools that can be used in the context of upstream bioprocess development. However, it is the individuals who utilize these tools who can elevate your process development to new heights. Through Eppendorf bioprocess trainings, you can drive improved outcomes and better performance in your laboratory by ensuring that your staff is well-prepared and understands their bioprocess systems and software in depth, enabling them to use these tools efficiently.
Efficiently advance from process development to production
The advancement of your process from R&D to production often includes an increase in working volume. Our solutions enable you to scale up your process while maintaining quality and yield. The Eppendorf portfolio of bioprocess controllers supports working volumes from 60 mL to 2,400 L, and many bioprocess systems have a wide working volume range for 10-fold scale-up and more on a single platform. We have also developed software to streamline the scale-up process. The Scale Up Assist software feature on selected systems assists you with the calculation of scale-up relevant process parameters transition from process development to production.
Our BioNsight cloud software enables you to easily compare behavior and performance of your processes at different scales and to transfer your data of your scale-up test runs to the cloud to leverage the power of AI-based analysis.
Our BioNsight cloud software enables you to easily compare behavior and performance of your processes at different scales and to transfer your data of your scale-up test runs to the cloud to leverage the power of AI-based analysis.
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Let’s talk development. Let’s talk progress. We're here to help you find inspiration to advance from R&D to production.
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I would like to learn more about how to scale-up my process.
Visit our website on bioprocessing scale-up to learn more about scale up strategies and to find some application examples of how Eppendorf customers used our products for bioprocessing scale up and scale down.
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I would like find out how to choose between batch, fed-batch, and perfusion and how to develop such processes.
Visit our website on bioprocess operation modes: batch, fed-batch, and continuous culture to learn more about the respective advantages and disadvantages, how to select the right process mode for your application, find out more about perfusion cell culture, and more.
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Secure your resources with successful batches
Batch failures can result in significant financial and time losses in upstream process development. Our bioprocess solutions adopt a multifaceted approach to mitigate risks. Our bioprocess systems are compatible with single-use bioreactors to minimize contamination and cross-contamination risks. Our bioprocess controllers and software offer precise control of your culture's growth environment and are designed to be user-friendly with built-in guided workflows to reduce handling errors. Our comprehensive training programs are designed to empower users, ensuring they can maximize the capabilities of bioprocess equipment.
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Let’s talk development. Let’s talk progress. We're here to help you find inspiration to ensure successful batches.
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I would like to gain insight into improving the reproducibility of my bioprocess.
Cells, culture medium, and a bioprocess control system all can be a source of variability and affect cell growth, viability, product formation, and product quality. In our ebook: Increasing the Reproducibility of Cell Culture Bioprocesses you find valuable information on how to identify factors which can contribute to inconsistent results and how to keep bioprocess conditions consistent between runs.
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Have you tested the biocompatibility of BioBLU Single-Use Bioreactors?
Yes, we have performed biocompatibility studies on CHO cell culture in BioBLU Single-Use bioreactors according to the DECHEMA recommendation for leachables studies . We found that during normal use there is no negative influence of the BioBLU material on CHO cell culture according to the DECHEMA guidelines. Find all the details in our application note .
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Single-use technology is a valuable tool for ensuring the safety of batches.
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